Q Lab Exercise - Preparing & Observing a Wet Mount • Points 5 • Questions 5 • Time Limit 5 Minutes • Allowed Attempts 3 Instructions Overview The following exercise will test your ability to: • describe the steps in preparing a wet mount • describe the procedure of preparing a wet mount of cheek epithelial cells • recall the proper technique of focusing on a specimen with a compound microscope • recall the definition of working distance Requirements You will need to set aside 10 minutes at a laptop or desktop computer in a distraction free room for each attempt. Grading This assignment is graded. Each of 5 multiple questions is worth 1 point for a total of 5 points possible. As 3 attempts are given, only the highest score obtained will be recorded as a grade. Directions • For each of 5 multiple choice questions, choose the one alternative that best completes the statement. • Questions will be shown one at a time and you will be allowed to return to a previous question. • Once you have clicked on the "Submit Quiz" button, you will not be able to return to the quiz. However, you are given 3 attempts to complete the assignment. Feedback You will receive immediate feedback for each question after completion of the quiz. As you complete each attempt, make sure you read the comments that accompany each of your responses to each of the questions on the quiz. A correct answer will be confirmed with an explanation as to why it is the correct answer; likewise, an explanation will be given for each incorrect answer. Resources If you require further clarification, please refer back to the Lab - Preparing and Observing a Wet Mount page. 1. What is the correct sequence of steps in preparing a wet mount? 1. Add a drop of stain to the preparation. 2. View the preparation. 3. Apply cells to the slide. 4. Lower the coverslip. 2. in the cheek cell wet mount preparation, the cells ________________. 3. It is recommended that when preparing a wet mount, the coverslip be placed gently onto the surface of the cell preparation. If the coverslip is merely dropped onto the cell preparation, ____________. 4.You have just prepared a wet mount of epithelial cells of the lining of the cheek. You initially view the cells at low power and focus on the specimen using the coarse adjustment knob. You then switch the objective lens to high power. You should now use the _____________ to focus on the specimen. 5. When the cheek cells are viewed at low power (10X) and then at high power (40X), what happens to the working distance with increased magnification?

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